Formulation and Evaluation of Polyherbal gel containing Thunbergia erecta and Bryophyllum pinnatum extracts for Wound healing activity

Healing a wound is a complicated physiological procedure that entails intricate exchanges between various cellular and molecular components. Herbal remedies have been recognized for their potentially promoting wound healing, and Thunbergia erecta and Bryophyllum pinnatum have demonstrated promising therapeutic properties. The purpose of this study was to develop and assess a multiherbal gel containing extracts of Thunbergia erecta and Bryophyllum pinnatum for their wound healing activity. The phytochemical screening of Thunbergia erecta and Bryophyllum pinnatum indicated the existence of active metabolites like flavonoids, saponins, glycosides, phenols, proteins alongwith amino acid. The gel was evaluated for several physicochemical characteristics, like pH (ranged from 5.8-6.5), viscosity (Formulations 1- 1453, 2-1431, and 3-1486 cps), and stability. An assessment was conducted on the multiherbal gel's ability to heal wounds through in vitro as well as in vivo studies. Furthermore, the gel's antimicrobial properties were examined against E. coli (Formulation 3 showing the best zones of inhibition) using a well diffusion assay. To assess the gel's effectiveness in encouraging wound closure, tissue regeneration, and reducing inflammation via excision model, in vivo investigations were carried out on animal models. Results showed that wound contraction increased with higher concentrations of herbal extracts. The polyherbal gel shows promising wound healing activity, indicating potential for further development as a natural, effective therapeutic option, emphasizing the importance of integrating traditional knowledge with modern scientific approaches.