Investigating optimal conditions for direct red 37 biodegradation using Enterococcus innesii strain CV10

The study tested the decolorization of Direct Red 37 dye using Enterococcus innesii CV10, a potential dye-decolorizing bacterial isolate. The optimal conditions were a temperature of 37°C, an inoculum size of 1-mL, an age of 18 hours, a pH of 8, and a water bath. The decolorization became less pronounced with increasing dye concentration and almost nonexistent at certain values. Decolorization was also studied about certain metals, carbon and nitrogen sources, and dietary factors. Dye degradation is seen in the meta-ring cleavage, also known as Positive Rothera’s reaction.