Influenza vaccination and cytokine profile dynamics in young men in Primorsky krai

Seasonal vaccination against influenza is considered the most effective method of specific prevention of this infection. However, in recent years, the issue of non­specific, training effects of vaccines on the immune system has been widely studied. One of the mechanisms of implementation may be a change in the production of cytokines in vaccinated people. Purpose of the study: to study the dynamics of pro­inflammatory and anti­inflammatory cytokines before and after vaccination against influenza. Materials and methods : The study involved 75 young men, the average age was 18.7±0.8 years, who were vaccinated with the Sovigripp vaccine. Those included in the study had two blood samples: (1) – before the flu vaccination; (2) – one month after the flu vaccination. Serum levels of proand anti­inflammatory cytokines were determined by solid­phase enzyme immunoassay: IL1β, IL4, IL6, IL8, IL10, IL17, IFNγ, TNFα. The results were recorded using an automatic enzyme immunoassay analyzer. Statistical processing of the obtained results was carried out using the STATISTICA 10 software package. Results: In the analysis of cytokines before vaccination, an increase in IL8, IL17 and IL6 levels was detected in 100% of cases – in 76% of cases. Within the generally accepted norms, only TNFa was determined in 92% of the surveyed population. The remaining indicators were reduced: IFNγ – in 100%, IL1β and IL4 – in more than 80% and IL10 – in 50% of cases. After influenza vaccination, there was a noticeable tendency to decrease the levels of all cytokines compared to baseline values. Conclusion: The results of the study revealed features in cytokine levels before and after influenza vaccination in healthy young people, which were characterized by deviations from the norm in both directions.