Anti-inflammatory and skin improvement effect of a complex extract including Sophorae fruztus, Zingiber officinale, and Akebia quinata

The purpose of this study was to investigate the effect on skin barrier improvement and anti-inflammatory effects using CSP-811 and CSP-532 complex extracts mixed with 8:1:1 and 5:3:2 ratios of Sophorae fructus, Zingiber officinale, and Akebia quinata. The experiment was conducted by setting a concentration range that exhibited cell viability of more than 95% in HaCaT and RAW 264.7 cells. To determine the effect of skin barrier improvement, the expression levels of filaggrin, involucrin, claudin-1, and ZO-1 were measured using HaCaT cells. As a result, compared to the group treated only with P. acnes, CSP-811 and CSP-532 increased in a concentration-dependent manner. As a result of measuring the nitric oxide production inhibitory activity, CSP-811 and CSP-532 decreased in a concentration-dependent manner. At the final concentration of 1,000 μg/mL, both extracts showed an inhibitory effect of more than 35% compared to the lipopolysaccharide alone treatment group. Afterward, as a result of protein expression levels of inducible NOS and cyclooxygenase-2, confirmed at the final concentration of 1,000 μg/mL, that CSP-811 and CSP-532 decreased in a concentration-dependent manner. Therefore, it was confirmed that CSP-811 and CSP-532 complex extracts have skin barrier improvement and anti-inflammatory effects, and it is judged to have excellent potential for use as a functional material.