In Vitro Drug Release Study of Bisacodyl Enteric-Coated Tablet in Various Artificial Dissolution Media

This study aims to investigate the effect of dissolution media on the release of bisacodyl from an enteric-coated tablet. Genuine phosphate buffer pH 7.4 (phosphate buffer), rat cecal-containing phosphate buffer pH 7.4 (rat cecal), lactase-containing phosphate buffer pH 7.4 (lactase), probiotics-containing phosphate buffer pH 7.4 (probiotics), and probiotics & lactase-containing phosphate buffer pH 7.4 (probiotics-lactase) were used as the colon simulation media. The study was carried out in a modified type I dissolution test apparatus for 12 hours: 2 hours in HCl pH 0.1 M, 3 hours in phosphate buffer pH 6.8, and 7 hours on colon simulation media, sequentially. The sample aliquots were analyzed using a UV-Vis spectrophotometer. At the end of the dissolution period, the bisacodyl released from tablets in phosphate buffer, rat cecal, lactase, probiotics, and probiotics-lactase were, respectively, 42.095%; 85.835%; 70.594%; 92.832%; and 110.63%. The dissolution media, which contains probiotics and lactase, facilitated the highest bisacodyl release, followed by probiotics, rat cecal, lactase, and phosphate buffer. The drug-release profile among various dissolution media showed significant differences, with p-values < 0.05. It can be concluded that pH, microbial activity, and enzyme content significantly influence the release profile of bisacodyl from enteric-coated tablets.