Efficacy of Single Love Garlic Extract on Self Nanoemulsifying Drug Delivery System (SCGE-SNEDDS) on Cytokine Expression In Vitro

Single clove Garlic Extract (SCGE) is a potential agent for anti-inflammation, several bioactive are lipophilic, volatile. A novel drug delivery system is required to preserve the bioactive and enhance the solubility. Self Nanoemulsfying Drug Delivery System (SNEDDS) can load extract that formed from carrier oil (VCO), surfactant (Tween-80), and co-surfactant (Glycerol), SCGE-SNEDDS prepared by mixing the surfactant and co-surfactant and adding the carrier oil with SCGE (20 mg/ml). The characteristics response emulsification time in 73.82 ± 0.91 second, % transmittance value of 78.86 ± 0.23%, and pH of 6.83 ± 0.72. The average particle size was 0.46 ± 0.01, Z-Average 23.10 ± 0.69, and zeta potential -33.07 ± 0.45 mV. The toxicity assay was determined in the 3T3-L1 cell line using MTT. The dose SCGE-SNEDDS in rank 62.5, 125, 250, 500, 1000, 2000 and 4000 µg/mL. SCGE-SNEDDS have a good response, characteristics, proved to be non-cytotoxic, and high cell viability (83.3 %). SCGE-SNEDDS had potential effect on the expression of TNF-α, not potential decreases IL-1β and increases IL-10 cytokines in 3T3-L1 cells.