Optimization of Prebiotic Exopolysaccharide production from Probiotic Lactobacillus brevis using Taguchi Experimental Design

In the current work the Taguchi experimental design was applied to maximize the yield of exopolysaccharide (EPS) from Lactobacillus brevis. The Taguchi modelwas employed to reduce the time and experimental procedures needed to optimize the various factors influencing its manufacture. In addition to minimizing the number of trials and material requirements needed for EPS synthesis. An orthogonal array arrangement of L27(36) was carried out using six factors: the pH of the Lactobacillus brevis culture, the temperature (°C), the incubation time (h), the inoculum size (µl), the volume (ml), and the glucose (%), each at three levels. Our findings showed that the ideal incubation parameters were pH 6.5 for the Lactobacillus brevis culture, 20°C for the culture, 24 hours for the incubation, 150µl for the inoculum size, 5ml for volume, and 2% for glucose. These together resulted in the best EPS generation by Lactobacillus brevis. The Taguchi experimental models' optimization of EPS production highlighted several significant findings on the interplay of the various driving factors resulting in the optimum EPS output in a single experiment. It is possible to refine the approach and raise the EPS weight. Applying the Taguchi modelin the biosynthetic EPS pathway, the synthesis of EPS increased to 560 mg/L from the basal condition of 376mg/L.