Study of the content of phenolic compounds, antimicrobial and antioxidant properties of the herb Caltha palustris

The presented research contributes to solving the task of pharmaceutical science in finding new sources of biologically active substances, namely phenolic compounds and flavonoids, which are valuable as antioxidant and antimicrobial agents, as well as further development of new effective substances based on the complex of biologically active compounds of Caltha palustris. An important aspect of the research is that the Caltha palustris grass is a little-studied object, but it has considerable experience of use in ethnomedicine. The extract from Caltha palustris was prepared from dry crushed raw materials using the extractant - water-ethanol solutions of the appropriate concentration (20%, 40%, 70% and 90%) in the ratio of raw extractant 1:10 at room temperature by maceration for 1-3 days. The quantitative content of phenols and flavonoids in the obtained extracts was determined was studied by the spectrophotometric method. The highest content of phenolic compounds(50.51±0.01) and flavonoids (19.85±0.01) was observed in the extract obtained by maceration of Caltha palustris grass using an extractant - 70% water-ethanol solution. Antioxidant activity was determined using the DPPH radical (2,2-diphenyl-1-picrylhydrazyl) and the ABTS cation radical (2,2’-Azino-BIS-(3-ethylbenzthiazoline-6-sulfonic acid). It was found that 70% and 90% of water-ethanol extracts of Caltha palustris have the highest level of antioxidant action. 70% and 90% Caltha palustris extracts have high antioxidant properties in two indicators of oxidative stress, as they are able to reduce the formation of free radicals in proteins and lipids. 70% and 90% extracts of Caltha palustris on rat liver hepatocytes under conditions of initiation of free radical oxidation in vitro showed antioxidant properties in two indicators of oxidative stress, reducing the level of thiobarbituric acid -active products carbonyl groups of proteins by more than 50% compared to the control. The antimicrobial effect of Caltha palustris extracts was studied. The study of the antimicrobial effect of caltha palustris herb extracts by diffusion into Agar were determined by the presence of Culture growth retardation around the well into which the test extract was introduced. The antimicrobial effect of Caltha palustris extracts was studied. The best antimicrobial effect was shown by 90% extract (for Bacillus cereus 34, Caltha palustris extract 90% (MIC 1:32 (0.00045 g) and Pseudomonas putida PDR 182 (1:4 (0.0036 g) and Caltha palustris extract 70% ( for Candida albicans 139 1:8 (0.0032 g). It is advisable to expand its research on the content of other biologically active compounds and actually deepen the study of the content of phenolic compounds and flavonoids, using other methods and approaches.