Pharmacognostical parameters, sphytochemical screening, TPC, TFC and evaluation of in-vitro anti-oxidant activity of leaves of Cymbidium aloifolium L (SW)

Natural drugs are one of the most important needs in daily life and their history is as old as human civilization. Orchids are nature's most beautiful and widely dispersed flowering plant groupings. Cymbidium aloifolium (L) SW family (Orchidaceae) is an epiphytic herbaceous orchid. The orchidaceae family is one of the oldest and largest flowering plant families in the world, with over 800 genera and between 25000 and 35000 species. The current study aims to examine in vitro antioxidant activity from different solvent extracts of Cymbidium aloifolium (L) SW leaves. The examination was conducted by several factors, including pharmacognostical, physicochemical, and phytochemical details of the leaf extracts of Cymbidium aloifolium (L) SW, which plays a vital role in laying down standardization and pharmacopoeia parameters. The antioxidant capacity of various solvent extracts of Cymbidium aloifolium (L) SW leaf extract was investigated using ferric reducing antioxidant potential (FRAP) tests and DPPH radical scavenging activities. A microscopic investigation exposes the leaf's cellular structure. To standardize the aim, physicochemical investigations were established, including swelling index, fluorescence analysis, total ash, acid insoluble ash, water soluble ash, and loss on drying. Antioxidant potential was determined by comparing the results with ascorbic acid standard. Leaves of Cymbidium aloifolium (L) SW methanolic extract showed the best result, followed by chloroform, ethyl acetate, pet ether, and water. These findings support the identification of Cymbidium aloifolium (L) SW and quality control measures. Further investigation is required to identify the specific phytochemical and the way in which it generates antioxidant activity.