Chemometrics Analysis of Liver Serum FTIR Spectra on Effect of Sembalun arabica Coffee Treated Upon Rat Induced-high Fat Diet

Background: That consumption of a high-fat food has increased the occurrence of rotundity in latest time. Diseases known to be diet-related, such as type 2 diabetes, cardiovascular disease, and various cancers, as well as increased morbidity and mortality, can be attributed to rotundity and overweight. A natural medicine that is believed to reduce rotundity is Arabica coffee. Objective of this study was evaluated the effect of Sembalun arabica Coffee (SAC) treated on reducing liver serum fat on rat induced-high fat based on Fourier transform infrared spectroscopy (FT-IR) spectra evaluated and classified accordingly Principal Components Analysis (PCA) and Partial Least-Squares Discriminant Analysis (PLS-DA). The rats were separated into 6 control groups consisted of 3 control groups (normal, positive, and negative) and 3 SAC treatment groups with three dose levels (200, 400, and 600 mg/kg BW). All groups except normal, were given high fat diet for 24 days, followed by treatment according to group for 24 days. Liver serum was collected and analyzed using FT-IR followed PCA and PLS-DA analysis. The results of the PCA analysis showed that the dose of 200 was very close to the positive group, and the PLS-DA analysis confirmed the results of identifying the activity of SAC in reducing fat in rats. A dose of 200 is more effective than a higher dose. This shows the dosage level at which Arabica coffee can work more effectively.