Callistemon viminalis (Sol. ex Gaertn.) exhibiting anti-neuroblastoma activity via molecular interaction of ascorbic acid and 5-lipoxygenase: An in vitro and in silico study

Neuroblastoma is the extracranial of children characterized by diverse clinical manifestations. The present study aimed to investigate the anti-neuroblastoma activity of hydromethanolic extract of Callistemon viminalis (Sol. ex Gaertn.) flower. The anti-neuroblastoma activity of hydromethanolic extract of C. viminalis flower was analysed in SH-SY5Y cell line using MTT assay. The study also evaluated drug-likeness and pharmacokinetic properties of bioactive compounds of extract using SwissADME tool and conducted in silico analysis to determine the molecular interaction between ascorbic acid and 5-LO for explaining the anti-neuroblastoma effect. Significant cytotoxicity was observed in SH-SY5Y cells in a dose and time dependent manner. The ADMET properties of the analysed bioactive compounds of C. viminalis showed them as a suitable drug candidate with appropriate pharmacokinetic properties. In silico results revealed that ascorbic acid binds into the catalytic domain and interacted with Ser447, Tyr470 and Arg457 of 5-LO with a binding energy of -5.11 Kcal/mol. The total phenol and ascorbate content were found to be 18.71 mg CAE/gm and 16.8 mg/100gm of extract, respectively. Hydromethanolic extract of C. viminalis flower induced cytotoxic effect in human neuroblastoma SH-SY5Y cells via interaction of its constituent ascorbic acid with the 5-LO, one of the important mediators of Neuroblastoma.