Screening of Antimetabolites of Interest present in a Streptomycetal isolate using Analytical tools

Secondary metabolites are the biosynthetic products of actinomycetes cells, produced against the competitors present the same niche. The study reports about production of antimetabolites from soil isolated Streptomyces sp. (LMA2) co-incubated with respective bacterial candidates (E.coli and S.aureus). The antimetabolic products could be detected through contemporary analytical tools namely, UV-Visible spectroscopy, FT-IR and GC-MS. The survival modality conferred by both co-incubated partners was further substantiated by the subculture procedure. There were putative compounds present in co-cultured broth, absorbed at 281nm and 547nm wavelengths fixed (UV-visible) for the vancomycin and azithromycin against Gram-positive and Gram-negative bacteria respectively. The wave numbers (cm-1) as detected from the respective FTIR analysis, were analyzed, referred with standard FTIR table and the putative functional groups were noted. From the GC-MS data recorded for cell free extracts of LMA2, there were compounds with noticeable biological activity. It was inferred that indole secreted by Streptomycetal isolate was the major compound with absolute percentage could be a potential active constituent to inhibit co-cultured bacterial growth.