Exploring the impact of Chlamydomonas reinhardtii Artificial Culture Medium on Ammonium and Phosphate Removal in Aquaculture Wastewater

This study utilized Chlamydomonas reinhardtii, an algal strain isolated from shrimp aquaculture wastewater ponds in Ca Mau province to investigate its capacity for ammonium and phosphate removal from wastewater. Cultivation occurred in both Tris-Phosphate-Acetate (TAP) and Bold's Basal Medium (BBM) under controlled conditions: a temperature of 25 ± 1 0C and a 24-hour light cycle provided by light-emitting diodes (LEDs). Over 28 days, four different concentrations of ammonium and phosphate were tested. Results demonstrated that algal biomass in the BBM medium exceeded threefold. Optimal algal biomass was observed at approximately 1.0 mg N.L⁻¹ ammonium concentration and about 5.0 mg P.L⁻¹ phosphate concentration. Notably, in the BBM medium, ammonium levels decreased rapidly within 7 days at a concentration of 4.0 mg N.L⁻¹ whereas in the TAP medium, it required 14 days. Conversely, phosphate concentration decline commenced after 14 days in both algal culture environments.