Apigenin in Apigenin Nanoliposomes: Development and Validation of an Analytical Technique Based on HPTLC

Apigenin is a flavonoid found in various fruits and vegetables, aimed at its extensive array of biological actions such as anti-oxidant, anti-viral, antibacterial, anti-inflammatory, including chemo preventive property. Therefore, this study aimed to develop, describe and evaluate apigenin nanoliposomes. In this study, ethanol injection method was employed to formulate apigenin nanoliposomes. The quantification of apigenin in the nanoliposomes was carried out using a HPTLC analysis technique. The defined standards were followed in development and validation of the approach based on ICH guidelines. An aluminium plate 60 F254 of silica gel was used as the stationary phase, toluene, ethyl acetate and acetic acid ratio of 6:3:1 (v/v/v) was the mobile phase. Validation was done on the linearity, range, detection limit, quantification limit, precision and robustness of the method. Reference standard for apigenin varied between 0.2-1 μg/band and a correlation value of 0.989 was observed. The results showed that the LOQ was 0.29 μg /band and LOD was 0.095 μg /band. % RSD readings for intraday and interday precision were determined to fall within the necessary range of 2 for the apigenin standard. The established analytical method for assessing apigenin in apigenin-loaded stealth liposomes has proven to be straightforward, reliable, accurate and robust method.