Diffractive Optical Elements for Three-Dimensional Nanoscopy Using Rotating Light Fields
The authors study diffractive optical elements manufactured via contact printing on a dichromated gelatin and direct laser writing on photoresist. The elements modify the point-spread function for three-dimensional super resolution fluorescence microscopy. It is shown that both elements produce two-lobed rotating light fields that can be used for three-dimensional nanoscopy. Results from the subdiffractive localization of fluorescent labels are presented, and the accuracy of coordinate reconstruction is determined.
Authors
- Д. В. Прокопова (ORCID: https://orcid.org/0000-0002-6955-8390)
- А. В. Наумов (ORCID: https://orcid.org/0000-0001-7938-9802)
- Dmitrij A. Belousov (ORCID: https://orcid.org/0000-0002-4284-5576)
- С. П. Котова (ORCID: https://orcid.org/0000-0003-2865-333X)
- Ivan Yu. Eremchev (ORCID: https://orcid.org/0000-0002-2239-5176)
- Sergey K. Golubtsov
- N. N. Losevsky
Institutions
- P.N. Lebedev Physical Institute of the Russian Academy of Sciences (RU)
- Russian Academy of Sciences (RU)
- Institute of Spectroscopy (RU)
- Institute of Automation and Electrometry (RU)
Publication Details
- Journal
- Bulletin of the Russian Academy of Sciences Physics
- Published
- 2024-12-01
- DOI
- https://doi.org/10.1134/s1062873824708390
- Citations
- 4
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 2.65