Molecular detection and identification of Enterobacter cloacae bacteria that cause urinary tract infection by PCR sequence technique

Aims: Enterobacter cloacae are considered Enterobacteriaceae that cause many diseases as well as urinary tract infections (UTI), respiratory infections and even wound infections.This research aimed to specify E. cloacae mainly based on PCR, the traditional bacterial technique reaction (PCR).Methodology and results: One hundred clinical samples were collected from patients suffering from different infections (urinary tract infections, wound infections, lesion infections and diabetic foot infections from Al-Salam and Al-Khansaa teaching hospitals.Twenty-five bacterial isolates belong to the bacterial type E. cloacae.These bacterial isolates were diagnosed based on both the VITEK 2 and PCR techniques.In addition, two bacterial isolates were diagnosed and registered in the NCBI-GenBank using a PCR technique based on the 16S rRNA gene. Conclusion, significance and impact of study:The matching ratio between the two local isolates recorded above with the same bacterial species worldwide were determined.The evolutionary relationships were determined through an explained evolutionary tree.Last, the types of mutations for the bacterial isolates were determined against the study by comparing the standard bacterial isolates.