Molecular detection of β-lactamase genes in Gram-negative bacteria isolated from cancer patients with urinary tract infections

Aims:The study sought to identify -lactamase genes in Gram-negative bacteria isolated from cancer patients' urinary tract infections.Methodology and results: One hundred urine samples were collected from cancer patients with UTI from 1 st November 2021 to 1 st February 2022.To identify broad-spectrum -lactamase enzymes, the disc approximation approach was applied.Conventional PCR detected -lactamase genes (TEM, blaoxa23, blaSHV and blaTEM).Escherichia coli had the most significant prevalence of UTIs (48.38%) among the bacteria studied (Pseudomonas aeruginosa 19.38%, Klebsiella pneumonia 12.9%, Proteus mirabilis 9.67% and Burkholderia cepaica 9.67%).Extended-spectrum beta-lactamase enzymes (ESBLs) were produced at a rate of K. pneumoniae 50%, E. coli 40%, P. mirabilis 33.3%, P. aeruginosa 16.66% and B. cepaica 0%.The detection rate of the TEM gene in E. coli isolates was 75%, while the detection rate of the blaoxa23 gene in P. aeruginosa isolates was 100%.Furthermore, the blaSHV gene of K. pneumoniae was found in 100% of cases.Finally, the blaTEM gene was thoroughly examined in P. mirabilis 100%.Conclusion, significance and impact of study: Escherichia coli was the most common bacterial species responsible for UTIs, followed by the other species.K. pneumoniae produced the most excellent broad-spectrum -lactamase enzyme of any Gram-negative species.