Optimization of a micro-scale air–liquid-interface model of human proximal airway epithelium for moderate throughput drug screening for SARS-CoV-2
This study demonstrates that the 96-well ALI model of primary human mucociliary epithelium can recapitulate the heterogeneity of infection among different donors and SARS-CoV-2 variants and can be used for moderate throughput screening. Compounds that target host factors showed variability among patients in response to SARS-CoV-2, while direct-acting antivirals were effective against SARS-CoV-2 despite the heterogeneity of patients tested.
Authors
- Laura Riva (ORCID: https://orcid.org/0000-0003-2414-7341)
- Gustavo Garcia (ORCID: https://orcid.org/0000-0002-1649-9251)
- Abdo Durra (ORCID: https://orcid.org/0000-0002-8738-9674)
- Arunima Purkayastha
- Tammy M. Rickabaugh
- Case W. McNamara (ORCID: https://orcid.org/0000-0002-5754-3407)
- Robert D. Damoiseaux (ORCID: https://orcid.org/0000-0002-7611-7534)
- Maisam Ghannam
- Arjit Vijey Jeyachandran
- Robert Garces
- Adam Aziz (ORCID: https://orcid.org/0000-0003-4992-1962)
- Vaithilingaraja Arumugaswami (ORCID: https://orcid.org/0000-0002-6872-5118)
- Brandon Han
- Melanie G. Kirkpatrick (ORCID: https://orcid.org/0009-0008-4335-5342)
- Ulrich Betz (ORCID: https://orcid.org/0000-0002-2355-7974)
- Brigitte N. Gomperts (ORCID: https://orcid.org/0000-0002-1809-723X)
- Chandani Sen (ORCID: https://orcid.org/0000-0003-4112-7473)
- Constance Yuen (ORCID: https://orcid.org/0000-0001-9714-9001)
- Amal Gebara-Lamb (ORCID: https://orcid.org/0009-0003-2271-3116)
- Felix W. Boulton
- Kristen Castillo
- Eugene L. Piatnitski Chekler
- Karen C. Wolff
Institutions
- California NanoSystems Institute (US)
- University of California, Los Angeles (US)
- Merck KGaA, Darmstadt (Germany) (DE)
- UCLA Health (US)
- UCLA Medical Center (US)
- Torrey Pines Institute For Molecular Studies (US)
- Ono Pharmaceutical (United States) (US)
- Mattel Children's Hospital (US)
- California Institute for Biomedical Research (US)
- Broad Center (US)
- UCLA Jonsson Comprehensive Cancer Center
Publication Details
- Journal
- Respiratory Research
- Published
- 2025-01-16
- DOI
- https://doi.org/10.1186/s12931-025-03095-y
- Citations
- 3
- Primary Topic
- Inhalation and Respiratory Drug Delivery
- Type
- article
- Field-Weighted Citation Impact
- 4.56
Funders
- U.S. Department of Defense
- Bill and Melinda Gates Foundation
- Tobacco-Related Disease Research Program
- California Institute of Regenerative Medicine
- Merck KGaA
- Fast Grants
- National Institutes of Health
- Office of the President, University of California
- National Cancer Institute
- National Institute of Allergy and Infectious Diseases
- National Center for Advancing Translational Sciences
- Clinical and Translational Science Institute, University of California, Los Angeles