Plant extracts inhibiting mycelial growth of Fusarium solani f. sp. piperis and Phytopythium helicoides

Mixtures of compounds with antifungal effect may increase the efficiency of products against fungi.The objective of this work was to evaluate the effect of different formulations based on coffee extracts, neem extracts and coffee pyroligneous extracts on the inhibition of Fusarium solani f. sp.piperis and Phytopythium helicoides mycelial growth.Three formulations with different concentrations of the extracts plus technical glycerin at 2.5; 5; 10; 15; and 20 mL L -1 , previously sterilized at 120C for 20 minutes, were added in PDA (Potato-Dextrose-Agar) culture medium.Discs containing culture medium with mycelium of F. solani f. sp.piperis or P. helicoides were transferred to the center of Petri dishes containing the treatments.After an incubation period of three days for P. helicoides and seven days for F. solani f. sp.piperis the diameter of the colonies were measured.In general, highest concentrations of the extracts resulted on inhibition of mycelial growth up to 100% for both microorganisms.