Identification of novel KRASG12D neoantigen specific TCRs and a strategy to eliminate off-target recognition
TCRs targeting the KRAS<sup>G12D</sup> peptide could be isolated from naive T cell repertoires. Integrating the TCR-peptide-HLA complex structure with a mammalian TCR library system could serve as a functional strategy to reduce potential TCR cross-reactivity with self-antigens, such as SMC1A<sub>29-38</sub>. Our findings evidenced an operable method to enhance TCR peptide specificity, while maintaining advanced functional avidity and potent anti-tumor activity.
Authors
- Aishun Jin (ORCID: https://orcid.org/0000-0001-9746-4220)
- Xiaxia Han (ORCID: https://orcid.org/0009-0008-1410-8609)
- Siyin Chen
- Shengchun Liu (ORCID: https://orcid.org/0000-0002-9933-3643)
- Xiaojian Han (ORCID: https://orcid.org/0000-0003-0477-7522)
- Meiying Shen (ORCID: https://orcid.org/0000-0001-8040-2330)
- Lin Zou (ORCID: https://orcid.org/0009-0004-0401-161X)
- Tong Chen (ORCID: https://orcid.org/0000-0002-8319-1142)
- Li Luo (ORCID: https://orcid.org/0000-0002-3876-882X)
- Wang Wang (ORCID: https://orcid.org/0000-0002-5549-2706)
- Jingjing Huang (ORCID: https://orcid.org/0000-0002-0912-534X)
- Yanan Hao
- Bozhi Wang (ORCID: https://orcid.org/0000-0003-1631-885X)
Institutions
- The Affiliated Yongchuan Hospital of Chongqing Medical University (CN)
- Chongqing Medical University (CN)
Publication Details
- Journal
- Journal of Translational Medicine
- Published
- 2025-01-16
- DOI
- https://doi.org/10.1186/s12967-025-06094-1
- Citations
- 3
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 4.08
Funders
- National Natural Science Foundation of China
- Chongqing Medical University