Crude oil degrading ability and optimization of Bacillus velezensis isolated from crude oil contaminated soil

Oil pollution is the largest serious threat to biological species.Crude oil has severe implications for creatures of all kinds.Every year, around 6 million tonnes of crude oil enter the environment, creating health risks to living things and causing environmental degradation.The greatest and most effective methods for recovering toxins in soil and water are found to be bioremediation.The present study focuses on the isolation, growth parameters optimization, molecular identification and degrading ability of bacteria that can degrade crude oil from soils collected from an automobile service center at Namakkal, Tamil Nadu.The higher significant crude oil degrading strain was isolated, and identified by screening test and 16s rRNA sequencing.technique.Cultural conditions including pH, temperature, crude oil concentration, inoculum concentration, and nitrogen source were optimized and it was statistically analyzed.Thirty-two bacterial strains were isolated using the enrichment method and the isolate named PS6 utilized crude oil in the highest amount during screening in comparison with other bacterial strains investigated in this present study.Noticeable degradation of crude oil was observed by GC-MS analysis of PS6 treated crude oil on comparing the peaks with the control.From 16s rRNA sequencing analysis, the isolated strain was identified as Bacillus velezensis.In statistical optimization, low p<0.0001values were observed.As a result, the isolated strain can be used in the bioremediation to clean up the environment.