Analyzing the minerals and phytochemicals composition of Phaseolus vulgaris L. using ethanol extract by gas chromatography and mass spectroscopy (GC-MS) analysis

The study employed a randomized complete block design with three replications, and included forty-three genotypes and one standard check (TKDPV(P) 13 from TKDPV(P) 1 to TKDPV(P) 44.The results showed that the genotype TKDPV(P) 8 showed the highest levels of crude protein (25.98%), crude fibre (6.53%), Fe (10.02 ppm), Zn (5.02 ppm), Cu (5.44 ppm), and Mn (2.21 ppm).Major bioactive compounds identified from the genotype TKDPV(P) 8 includes stigmasterol (19.02%), chondrillasterol (19.02%), eicosane (7.40%), phenol (6.29%), ethyl 3-hydroxybenzoate (6.29%), myristoyl chloride (5.79%), 1,3,12-nonadecatriene (3.68%), 15-hydroxypentadecanoic acid (3.63%), isopropyl linoleate (3.46%), n-methyl-1adamantaneacetamide (2.92%), 9,12,15-octadecatrienoic acid, ethyl ester (Z, Z, Z) (2.32%), pyrazine (2.18%), cyclohexane (1.80%), oxazole (1.79%), phthalic acid (1.70%), phytol (1.69%), 2-diamino-3,4,6trifluorobenzene (1.37%), benzenemethanol (1.35%), morpholine (1.23%), benzoic acid (0.60%), and pyrocatechol (0.41).Among forty-three genotypes and one standard check, the genotype TKDPV(P) 8 is deemed the highest mineral content, and enhancing phytochemical quality of P. vulgaris.