Validated RP-HPLC method for estimation of azilsartan medoxomil in bulk and tablet dosage form: A green analytical approach

An efficient, sensitive, and eco-friendly reverse-phase high-performance liquid chromatography ( RP-HPLC) method has been developed and validated for the quantification of azilsartan medoxomil, a widely used antihypertensive drug.Azilsartan medoxomil, known for its distinctive molecular structure and pharmacological efficacy in treating hypertension, necessitates a robust analytical approach for its accurate determination in bulk and tablet formulations.The proposed method was validated in compliance with ICH guidelines.The chromatographic separation was achieved using an Intersil C18 column (250 mm 4.6 mm, 5 m particle size) and a mobile phase comprising acetate buffer (pH 6.0) and acetonitrile in an 80:20 (v/v) ratio, operated at a flow rate of 1.0 ml/min.Detection was performed at 248 nm using a UV detector.The method exhibited linearity within the concentration range of 10-30 g/ml, with a correlation coefficient of 0.999.Precision was confirmed through low relative standard deviations (RSD) for both intra-and inter-day analyses.Robustness testing demonstrated the method's reliability under minor variations in chromatographic conditions.The validated RP-HPLC method was effectively applied for the analysis of azilsartan medoxomil in commercial tablet formulations.This method represents a reliable, eco-friendly, and efficient tool for routine quality control of azilsartan medoxomil in the pharmaceutical industry.