Comparative Study of Lipid Profile for Mice Treated with Cyclophosphamide by HPLC-HRMS and Bioinformatics
<b>Purpose:</b> Immunodeficiency conditions, which are characterized by reduced immune activity that promotes the development of chronic diseases, are needed for efficient monitoring. A promising area of monitoring and early diagnosis of immunodeficiency diseases is the determination of metabolic biomarkers in the blood. <b>Methods:</b> In this work, we identified a set of lipid biomarkers of immunodeficiency states by performing high-performance liquid chromatography-high-resolution mass spectrometry (HPLC-HRMS) analysis of blood plasma samples from mice and processing them with bioinformatics approaches. Potential biomarkers were selected through statistical analysis and further validated by MS/MS. <b>Conclusions:</b> As a result, 15 lipids were confirmed and selected as potential biomarkers of immunodeficiency states. The selected biomarkers can be further studied and serve as promising targets for the early diagnosis of immunodeficiency diseases.
Authors
- Elena Mukhlynina (ORCID: https://orcid.org/0000-0002-5159-4465)
- Aroa López-Santamarina (ORCID: https://orcid.org/0000-0002-2159-1474)
- Olga V. Berdiugina (ORCID: https://orcid.org/0000-0003-3479-9730)
- Elena G. Kovaleva (ORCID: https://orcid.org/0000-0002-3111-345X)
- Alberto Cepeda (ORCID: https://orcid.org/0000-0002-9324-1342)
- Fernando Polanco Espino (ORCID: https://orcid.org/0000-0001-5191-3190)
- Ekaterina Demicheva (ORCID: https://orcid.org/0000-0003-0744-5494)
- Вадим А. Шевырин (ORCID: https://orcid.org/0000-0002-0369-0786)
- Alicia del Carmen Mondragón (ORCID: https://orcid.org/0000-0003-1274-5539)
- Alejandra Cardelle‐Cobas (ORCID: https://orcid.org/0000-0002-1271-513X)
- Olga Solovyova (ORCID: https://orcid.org/0000-0003-1702-2065)
- José Manuel Miranda (ORCID: https://orcid.org/0000-0001-7992-1491)
- И. Г. Данилова (ORCID: https://orcid.org/0000-0001-6841-1197)
- Aleksey Buhler
- Pavel A. Vedeneev
Institutions
- Ural Federal University (RU)
- Universidade de Santiago de Compostela (ES)
- Institute of Immunology and Physiology (RU)
Publication Details
- Journal
- Metabolites
- Published
- 2025-01-16
- DOI
- https://doi.org/10.3390/metabo15010060
- Citations
- 1
- Primary Topic
- Metabolomics and Mass Spectrometry Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.81