Probing Native CB<sub>2</sub> Receptor Mobility in Plasma Membranes of Living Cells by Fluorescence Recovery After Photobleaching

In this study, we employed a novel fluorescent probe, RO7304924 - which selectively targets cannabinoid 2 receptor (CB<sub>2</sub>R) - to assess the lateral mobility of CB<sub>2</sub>R within the plasma membrane of Chinese hamster ovary cells stably expressing a functional, untagged receptor variant. Utilizing confocal fluorescence recovery after photobleaching (FRAP), we quantified the diffusion coefficient and mobile fraction of CB<sub>2</sub>R, thereby demonstrating the efficacy of RO7304924 as an innovative tool for elucidating the dynamics of this major endocannabinoid-binding G protein-coupled receptor. Our present findings highlight the potential of combining advanced ligand-based fluorescent probes with FRAP for future investigations into the biochemical details of CB<sub>2</sub>R mobility in living cells, and its impact on receptor-dependent cellular processes.

Authors

Institutions

Publication Details

Journal
ChemBioChem
Published
2025-01-16
DOI
https://doi.org/10.1002/cbic.202400921
Primary Topic
Pancreatic function and diabetes
Type
article
Field-Weighted Citation Impact
0.00
Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

Probing Native CB2 Receptor Mobility in Plasma Membranes of Living Cells by Fluorescence Recovery After Photobleaching

Alessandro Leuti, Francesca Ciaramellano, Erick M. Carreira, Alexandrine D. E. Kurtz et al.
ChemBioChem
Pancreatic function and diabetes
article

Probing Native CB2 Receptor Mobility in Plasma Membranes of Living Cells by Fluorescence Recovery After Photobleaching

Alessandro Leuti, Francesca Ciaramellano, Erick M. Carreira, Alexandrine D. E. Kurtz, Patrick Pfaff, Uwe Grether, Matthias V. Westphal, Sergio Oddi, Roman C. Sarott, Mauro Maccarrone
article en

Abstract

In this study, we employed a novel fluorescent probe, RO7304924 - which selectively targets cannabinoid 2 receptor (CB<sub>2</sub>R) - to assess the lateral mobility of CB<sub>2</sub>R within the plasma membrane of Chinese hamster ovary cells stably expressing a functional, untagged receptor variant. Utilizing confocal fluorescence recovery after photobleaching (FRAP), we quantified the diffusion coefficient and mobile fraction of CB<sub>2</sub>R, thereby demonstrating the efficacy of RO7304924 as an innovative tool for elucidating the dynamics of this major endocannabinoid-binding G protein-coupled receptor. Our present findings highlight the potential of combining advanced ligand-based fluorescent probes with FRAP for future investigations into the biochemical details of CB<sub>2</sub>R mobility in living cells, and its impact on receptor-dependent cellular processes.

ChemBioChemVol. 26(8)
Roche (Switzerland) (CH), University of Teramo (IT), Università Campus Bio-Medico (IT), University of L'Aquila (IT), ETH Zurich (CH), European Brain Research Institute (IT), Fondazione Santa Lucia (IT)
Openalex Percentile: Top 99%
Pancreatic function and diabetes
DOIOpen Access PDFOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.

Probing Native CB<sub>2</sub> Receptor Mobility in Plasma Membranes of Living Cells by Fluorescence Recovery After Photobleaching — Alessandro Leuti, Francesca Ciaramellano, et al. · ChemBioChem (2025) | TGRS Research Map | TGRS