Preparation of canine platelet rich fibrin membrane and its characterisation using light microscopy and scanning electron microscopy

The current study was undertaken to analyse the cellular components and three-dimensional organisation of “platelet rich fibrin” membrane (PRFM), an autologous blood derived biomaterial. Ten millilitres of whole blood collected from six canine patients which was then centrifuged at 542.4G (3200 rpm for 10 minutes). The PRF clot thus obtained was pressed to form the platelet rich fibrin membrane (PRFM). The structural analysis and cell composition of PRFM was analysed using scanning electron microscopy (SEM) and histopathology. Evaluation with SEM revealed clearly organised fibrin in parallel strands and the cells were embedded in this compact fibrin matrix. Histopathological examination showed that the leukocyte cytoplasm stained a darker pink along with RBCs, the fibrin matrix seemed homogenous in light pink and platelet aggregates were dark blue/violet. According to the study, the majority of the platelets and leukocytes from the blood sample were present in the PRFM, and their distribution followed a characteristic three-dimensional pattern with the fibrin strands. Keywords: PRF, SEM, dogs, keratitis