Determination of the Content of FAD Cofactor and NAD(P)H-Oxidase Complexes in Mouse Splenocytes and Lewis Carcinoma Cells Under Conditions of Apoptosis by Confocal Microscopy Method

In this paper, using fluorescence and confocal microscopy, we study the content of the flavin adenine nucleotide (FAD) cofactor and enzymatic NAD(P)H-oxidase complexes (with fluorophores Annexin V‑ FITC, 7-AAD (7-aminoactinomycin D), EtBr) under conditions of apoptosis caused by sodium anphen with hydrogen peroxide in healthy mouse splenocytes and Lewis carcinoma tumor cells. The use of fluorescence microscopy allows observing and quantifying the apoptotic effect of sodium anphen and hydrogen peroxide, and it also allows visualizing the metabolic changes in the cell, including the increased fluorescence of FAD in tumor cells and NAD(P)H-oxidase complexes in splenocytes. The data obtained indicate the possibility of using sodium anphen in combination with hydrogen peroxide as an antitumor drug acting on certain types of cells.