The proposed protein and carbohydrate ingredients and the acceptance of moist feed by newly hatched Bigfin reef squid (Sepioteuthis lessoniana)

Bigfin reef squid ( Sepioteuthis lessoniana ) begin to consume non-living food at least 20 days after hatching while they do not accept artificial diet during early phase of post-hatching. This prevents feasibility from economical aquaculture for this squid. The same situation exists for other squids as well. Here, thirty sources of protein and seven of carbohydrate were tested for in vitro digestibility using digestive enzymes from viscera of Bigfin reef squid. Suitable protein ingredients included the meat of blue swimming crab, catfish, emperor fish, Indian anchovy, Indian mackerel, Nile tilapia, Pacific white shrimp, striped catfish, trash fish and yellow-stripe scad. Suitable carbohydrate sources were corn starch, potato starch and rice flour. These ingredients might be included in an artificial diet for rearing Bigfin reef squid. Based on in vivo preliminary investigation, we first report eight-day-old Bigfin reef squid accepted moist feed produced from red emperor fish ( Lutjanus sebae ), striped catfish ( Pangasianodon hypophthalmus ) and Pacific white shrimp ( Litopenaeus vannamei ) in a 1:1:1 (w/w/w) ratio. The squid accepted the feed on the first day of training. Subsequently, the rate of acceptance increased, and reached 100 % on day 4. Latency-to-seizure of feed decreased on days 3 and 4 relative to the first two days of training. Based on our investigations, feeding Bigfin reef squid by moist feed might be possible. Considering the unique feeding habits compared to other cephalopod species, tailored feeding programs would be required. • Newly hatched squid accepted non-living feed on the first day of training. • Feed acceptance reached 100 % on day 4. • Ten of thirty protein sources were suitable for bigfin reef squid. • Corn starch, potato starch and rice flour were suitable carbohydrate sources.