Extensive Diagnosis of Chronic Brucellosis Using Interferon -Y

The study was carried out at Anbar Teaching Hospitals from October 2022 to September 2023 and included collect of 86 individual samples (54 males and 32 females) then, examined by using the Rose Bengal test to identify those infected with brucellosis. All individuals were divided into two groups, which included the control group (N= 44) who tested negative for the Rose Bengal test, and the patient's group (N= 42) tested positive for the Rose Bengal test and was suspected of having brucellosis. Brucella diagnosis by interferon-γ release assay (IGRA) was used to investigate the ability of Brucella infection to stimulate IFN-γ and compared it with the results of the Rose Bengal test. IGRA diagnosis was performed on 24 rose-Bengal test-positive blood samples isolated from the group of patients suspected of having Brucella infection and on two blood samples from the control group. Qualitative detection of Brucella-specific IgG antibodies by ELISA in the serum of all research participants. A molecular diagnosis was performed using a PCR amplification program to detect 16SrRNA genes for 28 blood samples isolated from the group of patients suspected of being infected with Brucella and for two blood samples from the control group. Finally, Analysis of data was carried out using the available statistical package of IBM SPSS-29.