Green synthesis of silver nanoparticles using Annona muricata extract and their antibacterial and antibiofilm activity against multidrug-resistant bacteria

The study is centered on the synthesis, characterization, and application of silver nanoparticles (Ag NPs), which show promising potential for biomedical applications. Nanobiotechnology allows for broader application while reducing the reliance on expensive and hazardous chemicals through the sustainable production of nanoparticles from plant resources. This research aims to develop an environmentally safe process for producing silver nanoparticles (Ag NPs) using the Annonaceae fruit's aqueous extract as both a capping and reducing agent, and to assess their potential pharmaceutical applications as antibacterial and antifungal agents. Ag NPs were characterized using UV–vis spectroscopy technique, FT-IR, field emission scanning electron microscopy (FESEM), AFM and X-ray diffraction (XRD). The biological activity of Ag NPs was investigated against two genera of pathogenic bacteria, Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus. using the agar well diffusion assay. This study includes the plant's possible use in the pharmaceutical industry. The plant's nutritious fruit extract opens the door to further research into possibly using it as an edible medicine.