PRODUCTION AND OPTIMIZATION OF PHYTASE BY ASPERGILLUS NIGERUSING SUBMERGED FERMENTATION

Phytases are the enzymes hydrolyzing phytic acid to less phosphorylated myo-inositol derivatives, releasing inorganic phosphate. Phytase has become an important industrial enzyme and is the object of extensive research. The present article implies an extracellular phytase production by Aspergillus niger(b2) under submerged fermentation. Physical and Chemical conditions tested for optimal production of phytase using the single variable mode optimization technique. A considerable higher phytase production was obtained using phytate sodium substrate (102.46 U/ml) at pH 5, on 5th day of incubation at 300C and Agitation 200 rpm. Glucose is considered as suitable carbon source whereas pepton is for nitrogen. The phytases produced can be used further for various applications. Keywords: Aspergillus niger, Submerged fermentations, phytase optimization.