Study on the prevention and treatment of noise induced hearing loss in mice with saturated hydrogen physiological saline

Objective: This experiment explored the preventive effect of saturated hydrogen physiological saline by establishing a mouse model of noise induced hearing loss. Methods: 24 C57/6J male mice (8W) with SPF level and normal ABR threshold were randomly divided into three groups: normal control group, noise group, and saturated hydrogen physiological saline group. The saturated hydrogen physiological saline group received daily intraperitoneal injections of saturated hydrogen physiological saline (1 ml/100g) three days before receiving steady-state noise exposure (110 dB SPL, 4h). Perform ABR audiometry before and 1, 3, and 7 days after noise exposure. After completion, the animals were euthanized and samples were collected for observation under scanning electron microscopy. Results: The ABR results showed that the hearing threshold of mice in the saturated hydrogen physiological saline group was lower than that of the noise group. Scanning electron microscopy observation showed that the cilia morphology of cochlear hair cells in the saturated hydrogen physiological saline group was better than that in the noise group. Conclusion: Daily intraperitoneal injection of saturated hydrogen physiological saline three days before noise exposure has a certain protective effect on hearing loss caused by steady-state noise, laying the foundation for further exploration of its specific mechanism at the pathological molecular level.