The Golgi complex governs natural killer cell lytic granule positioning to promote directionality in cytotoxicity
Cytotoxic immune cells mediate precise attacks against diseased cells to maintain organismal health. Their operational unit of killing and host defense is lytic granules (LGs), which are specialized lysosomal-related organelles. Precision in cytotoxicity is achieved by converging the many LGs to the microtubule-organizing center (MTOC) and polarizing these to the diseased cell for secretion. We identify unappreciated intimate relationships between the Golgi, MTOC, and LGs after cytotoxic cell activation, as well as the trans-Golgin protein GCC2 on the LG surface. GCC2 serves to tether LGs to the Golgi following convergence, and both GCC2 and the Golgi are required for the persistence of convergence. GCC2 allows LGs to utilize the Golgi as a docking station preventing LG dispersion and innocent bystander killing in complex three-dimensional environments. We also identify GCC2 variants causing human natural killer cell deficiency, further emphasizing the importance of LG convergence and Golgi linkage in precision targeting for human immunity.
Authors
- James R. Lupski (ORCID: https://orcid.org/0000-0001-9907-9246)
- Jennifer E. Posey (ORCID: https://orcid.org/0000-0003-4814-6765)
- Alexander Frumovitz (ORCID: https://orcid.org/0009-0003-3631-0502)
- Iván K. Chinn (ORCID: https://orcid.org/0000-0001-5684-5457)
- Yu Li (ORCID: https://orcid.org/0000-0002-5304-6454)
- Emily Margaret Mace (ORCID: https://orcid.org/0000-0003-0226-7393)
- Aaron Bodansky (ORCID: https://orcid.org/0000-0001-8943-8233)
- Everardo Hegewisch-Solloa (ORCID: https://orcid.org/0000-0002-0223-3516)
- K SHEEHAN
- Susan E. Prockop (ORCID: https://orcid.org/0000-0003-4175-5728)
- Evelyn Hernández (ORCID: https://orcid.org/0000-0001-8287-0015)
- Jordan S. Orange (ORCID: https://orcid.org/0000-0001-7117-7725)
- Luis Alberto Pedroza (ORCID: https://orcid.org/0000-0002-5687-8352)
- Frederique van den Haak (ORCID: https://orcid.org/0009-0002-4362-6444)
- Tabitha K Orange
Institutions
- Boston Children's Hospital (US)
- University of California, San Francisco (US)
- Baylor College of Medicine (US)
- General Electric (Spain) (ES)
- Baylor Genetics (US)
- Columbia University (US)
Publication Details
- Journal
- Cell Reports
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1016/j.celrep.2024.115156
- Citations
- 3
- Primary Topic
- Immune Cell Function and Interaction
- Type
- article
- Field-Weighted Citation Impact
- 2.39
Funders
- National Institutes of Health