Validated LC-MS/MS methodology for the quantification of CBD, trace level THCA and UK controlled cannabinoids (Δ⁹-THC, Δ⁸-THC, CBN and THCV) in food samples

Products containing cannabidiol (CBD) have become increasingly popular due to consumer-perceived benefits of improving health and well-being. More specifically in the United Kingdom (UK), CBD food products are categorised as <i>novel foods</i>. For these products to remain on the market, they must have authorisation from the Food Standards Agency on the basis that they are safe, correctly labelled, and do not contain substances classified under controlled drugs legislation in accordance with any existing or future Home Office guidance. This demands for analytical laboratories to be able to accurately measure the CBD concentration using validated methods to confirm correct labelling, as well as the controlled cannabinoid content to ensure the products comply with legislation. To address some of these challenges, this work describes two similar liquid chromatography tandem mass spectrometry (LC-MS/MS) methods which were developed and validated for measuring CBD and trace level controlled cannabinoids in CBD containing foods. The accuracy of the methods developed were tested for the first time through an interlaboratory comparison involving expert laboratories. The methods were applied to a comprehensive study of 148 CBD edible products. In 13 of the products tested (9% of the total) CBD was found below the limit of quantification. Of the remaining 135 products (91% of the total), 66% were found to have detectable amounts of one or more controlled cannabinoids. Of the 13 samples that did not contain detectable levels of CBD, two did contain quantifiable levels of controlled cannabinoids. The validation and sample analysis results reveal intriguing sets of data which help gauge the ongoing narrative surrounding CBD analysis and CBD products available in the UK. The research contributes to the global effort to keep unsafe products off the market.