The effect of disinfectant based on hydrogen peroxide, lactic and perlactic acids on the laboratory rats

Nowadays, there are many types of disinfectants available on the market. At the same time, an analysis of scientific literature shows the lack of disinfectants based on perlactic acid, which has no less pronounced oxidative properties than the other acids, used for disinfection. The aim of this research was to investigate the effect of “Biolide” disinfectant based on hydrogen peroxide, lactic and perlactic acids on the laboratory rats. Different concentrations of this disinfectant was inserted to animals intragastrically, by application on skin and by inhalation (cages processing). Authors identified the LD50, irritant and sensitizing effects, immunological parameters of blood. The LD50 was 5292 mg/kg for male and 5041 mg/kg for female rats after intragastric insertion. This disinfectant had a temporary irritant and sensitizing effects on the skin of rats and no negative effect on the indicators of nonspecific immune response in the form of 0.2 % solution. Studies regarding the SBA and the quantitative content of E and EAC rosette-forming cells (E-RFC and ЕАС-RFC) showed a suppressive effect of not only 2.0 % but also 1.0 % of the biolide solution. This study proved that biolide has no toxicity properties and exhibits temporary sensitizing and irritating effect only in the form of a concentrate. Results of research indicated that this disinfectant belongs to fourth class according to the classification of chemicals by the degree of hazard, which allows its use in the presence of animals.