Maternal Separation as Model Maternal Mortality Induced Caspase3 Expression of Cerebrum and Cerebellum in Rattus Norvegicus Newborn

Maternal mortality results from the breakdown of the bonding-attachment between mother and child, affecting the development of psychological factors and the expression of initiator-activating caspase-3.In this study, we described maternal separation from neonates using a posttest-only control group experimental design.The research sample was randomly split into two groups: Rattus norvegicus newborns aged 3 days and those that were not separated.The assessment employed immunohistochemistry and was evaluated using the Rammele Scale Index (Immunoreactive Score / IRS) analysis.These findings demonstrate a notably elevated expression of caspase-3 in the cerebrum of Rattus norvegicus neonates subjected to maternal separation therapy in comparison to the control group, attributed to neuropsychiatric disorders such as anxiety and depression in infants, as well as the prolonged impact of parental loss on neurodevelopmental and behavioral well-being, which may enhance susceptibility to psychopathology.Additionally, there are only minor variations in caspase-3 expression in the cerebrum of newborn Rattus norvegicus between those separated and not separated from their mother, influenced by the duration and frequency of separation.To our knowledge, there is no documentation of maternal separation that might trigger caspase-3 expression in the cerebrum and cerebellum.These findings present novel insights that could be significant for clinical applications in newborns.