THE EFFECT OF ADDING 25-HYDROXYCHOLECALCIFEROL TO THE DIET ON SOME BIOCHEMICAL CHARACTERISTICS OF JAPANESE QUAIL

This experiment was conducted in the quail breeding hall of the Department of Animal Production at the College of Agriculture / Anbar University for the period from 11/23/2022 until 1/25/2023 (8 weeks) with the aim of studying the effect of adding different levels of 25-hydroxycholecalciferol to the diet on some blood biochemical characteristics. For Japanese quail. 240 birds (180 females + 60 males) at the age of 45 days were used, from Japanese quail raised in the Department of Agricultural Research / Abu Ghraib. The birds were randomly distributed into 4 treatments, each containing 3 replicates, with 15 females and 5 males for each. Repeatedly, I raised birds in floor cages inside the hall, each cage with dimensions of 50 x 60 x 67 cm. The experimental treatments were distributed as follows: the first treatment, T1 / males and females eating a diet free of any additive (control diet). The second treatment, T2 / adding vitamin D3 at a rate of 50 mg/kg feed. The third treatment: T3 / adding vitamin D3 at a rate of 100 mg/kg feed. Fourth treatment: T4 / adding vitamin D3 at a rate of 150 mg/kg feed. The experimental results showed that there was a significant increase (0.05≥P) in the total protein concentration for treatment T2 when adding 50 mg/kg feed of vitamin D3 compared to the control group for the two periods, as well as a significant increase (0.05≥P) for the concentration of cholesterol compared with the control group for treatment T2 when Add 50 mg/kg of vitamin D3 to the feed for the two periods. The results also showed a significant increase in the concentration of HDL during the first period (0.05 ≥ P) of treatment T2 when adding 50 mg/kg feed of vitamin D3, and the average of the two periods (0.05 ≥ P) of treatment T2 when adding 50 mg/kg feed of vitamin D3 compared with the control group. the control. The results also indicate that there was a significant increase during the second period (0.05 ≥ P) for the enzyme ALT for treatment T4 when adding 150 mg/kg feed of vitamin D3 and a significant increase (0.05 ≥ P) for the enzyme AST for treatment T4 when adding 150 mg/kg feed of vitamin D3 compared to with the control group. While no significant differences were observed for albumin, calcium in blood serum and egg yolk