Microbial Load Analysis and Characterization of Drug-Resistant Bacteria from Raw Meats in Gopalganj District

Antimicrobial resistance is a significant threat to people and animals around the world. Microorganisms are becoming more resistant to antibiotics, according to researchers. This is because more antibiotics are being used on animals and fowl. The main goal of this study was to look at and explain the types of bacteria resistant to antibiotics found in raw beef in a lab setting. For this study, two pieces of raw beef meat were bought from local shops in Gopalganj, Bangladesh. Once the bacteria were grown and put on selective media, they were identified using Gram staining and different biochemical tests. They were also tested for drug resistance using standards from the Clinical and Laboratory Stan. The Kirby-Bauer disc diffusion method was utilized to conduct antibiotic susceptibility testing for eight antibiotics, namely Ampicillin (Amp), Amoxicillin (Amx), Azithromycin (Azm), Cefotaxime (CTX), Ciprofloxacin (CIP), Erythromycin (E), Streptomycin (S), and Kanamycin (K). The resistance or susceptibility patterns of Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae isolates were subsequently determined. The disc diffusion antimicrobial susceptibility assay showed that the S. aureus isolates were not susceptible to ciprofloxacin. The E. coli and K. pneumoniae isolates were not susceptible to ampicillin and amoxicillin, commonly used to treat severe bacterial infections in humans. These results show that eating tainted beef is terrible for your health and that there needs to be stricter rules about food safety, especially when it comes to using antibiotics on animals. The study stresses the importance of monitoring drug resistance in dangerous pathogens so that bacteria not susceptible to antibiotics don't spread.