Quantitative imaging of loop extruders rebuilding interphase genome architecture after mitosis
How cells establish the interphase genome organization after mitosis is incompletely understood. Using quantitative and super-resolution microscopy, we show that the transition from a Condensin to a Cohesin-based genome organization occurs dynamically over 2 h. While a significant fraction of Condensins remains chromatin-bound until early G1, Cohesin-STAG1 and its boundary factor CTCF are rapidly imported into daughter nuclei in telophase, immediately bind chromosomes as individual complexes, and are sufficient to build the first interphase TAD structures. By contrast, the more abundant Cohesin-STAG2 accumulates on chromosomes only gradually later in G1, is responsible for compaction inside TAD structures, and forms paired complexes upon completed nuclear import. Our quantitative time-resolved mapping of mitotic and interphase loop extruders in single cells reveals that the nested loop architecture formed by the sequential action of two Condensins in mitosis is seamlessly replaced by a less compact but conceptually similar hierarchically nested loop architecture driven by the sequential action of two Cohesins.
Authors
- Marko Lampe (ORCID: https://orcid.org/0000-0002-4510-9048)
- Hannah Pflaumer (ORCID: https://orcid.org/0000-0003-4927-0899)
- Andreas Brunner (ORCID: https://orcid.org/0000-0003-3136-6382)
- Aliaksandr Halavatyi (ORCID: https://orcid.org/0000-0002-9002-457X)
- Jan Ellenberg (ORCID: https://orcid.org/0000-0001-5909-701X)
- Jan‐Michael Peters (ORCID: https://orcid.org/0000-0003-2820-3195)
- Kai Sandvold Beckwith (ORCID: https://orcid.org/0000-0001-5598-5482)
- M. Julius Hossain (ORCID: https://orcid.org/0000-0003-3303-5755)
- Wanlu Zhang (ORCID: https://orcid.org/0000-0002-7119-1874)
- Natalia Rosalía Morero (ORCID: https://orcid.org/0000-0003-0092-5663)
Institutions
- European Bioinformatics Institute (GB)
- Research Institute of Molecular Pathology (AT)
- Norwegian University of Science and Technology (NO)
- Heidelberg University (DE)
- Science for Life Laboratory (SE)
- European Molecular Biology Laboratory (DE)
- University of Southampton (GB)
Publication Details
- Journal
- The Journal of Cell Biology
- Published
- 2024-12-03
- DOI
- https://doi.org/10.1083/jcb.202405169
- Citations
- 18
- Primary Topic
- Genomics and Chromatin Dynamics
- Type
- article
- Field-Weighted Citation Impact
- 4.21
Funders
- Human Frontier Science Program
- Alexander von Humboldt-Stiftung
- Boehringer Ingelheim
- Paul G. Allen Frontiers Group
- European Commission
- Boehringer Ingelheim Fonds
- Deutsche Forschungsgemeinschaft
- Vienna Science and Technology Fund
- Österreichische Forschungsförderungsgesellschaft
- National Institutes of Health
- European Research Council
- Common Fund