Unraveling the in vivo pharmacokinetic behavior of mPEG5-NH2 polymer in rats by UHPLC-MS/MS assay
As an important chemical reagent, methoxy polyethylene glycol amine (mPEG-NH<sub>2</sub>) is widely used in biomedical field. Unraveling the pharmacokinetic behavior of mPEG-NH<sub>2</sub> polymers is essential for revealing the toxicity and efficiency of mPEG-NH<sub>2</sub> related drug delivery systems. In this study, a simple analytical assay based on mass spectrometry (MS) was first established and validated for quantification of mPEG<sub>5</sub>-NH<sub>2</sub> in biological matrix. The multiple reaction monitoring (MRM) transitions at m/z 252.2 (precursor ions) → 87.7 (fragment ions) and m/z 371.2 (precursor ions) → 89.2 (fragment ions) were chosen to determine mPEG<sub>5</sub>-NH<sub>2</sub> and OH-PEG<sub>8</sub>-OH, respectively. The UHPLC-MS/MS assay showed excellent linearity over the range of 0.01-10 μg/mL. Intra-day and inter-day accuracies and precisions of the assay were all within ± 6.44 %. The analytical assay was successfully applied to reveal the in vivo pharmacokinetic behavior of mPEG<sub>5</sub>-NH<sub>2</sub> in rats.
Authors
- Chunpeng Feng (ORCID: https://orcid.org/0009-0004-3816-0306)
- Qingbin Wang (ORCID: https://orcid.org/0000-0002-2415-4122)
- Jie Tian (ORCID: https://orcid.org/0000-0003-0498-0432)
- Meichen Liu (ORCID: https://orcid.org/0000-0003-4536-5444)
- Lei Yin (ORCID: https://orcid.org/0000-0002-8068-5999)
- Xuan Zhao (ORCID: https://orcid.org/0000-0001-9225-8452)
- Yingxia Guo
Institutions
- Dalian Ocean University (CN)
- Dalian University of Technology (CN)
- Cell Technology (China) (HK)
- Beike Biotechnology (China) (CN)
Publication Details
- Journal
- Journal of Chromatography B
- Published
- 2025-01-10
- DOI
- https://doi.org/10.1016/j.jchromb.2025.124460
- Citations
- 1
- Primary Topic
- Nanoparticle-Based Drug Delivery
- Type
- article
- Field-Weighted Citation Impact
- 0.60
Funders
- Fundamental Research Funds for the Central Universities