Characterization of Zerumbone Loaded Chitosan-Folic Acid Nanoparticles and Its Cytotoxic Activity Against MCF-7 Cells

Zerumbone has the potential to be developed as an anticancer because it has a variety of effects on the proliferation, angiogenesis, and apoptosis of different cancer cell lines. Because of the well-known folate-receptor mediated endocytosis, folic acid-chitosan conjugated nanoparticles demonstrated much greater cell uptake capacity. Zerumbone will be described after being formed into a nanoparticle system using a chitosan carrier conjugated with folic acid, compared to zerumbone-loaded chitosan nanoparticles. Employing the MTT assay method, nanoparticles were prepared by ionic gelation and characterized based on particle size, polydispersity index, zeta potential, entrapment efficiency, drug loading, release test, and cytotoxic test MCF7 cells. The synthesized nanoparticles had a zeta potential of -37.06, a PDI value of 0.32, and an average particle size of 195.13 nm. The percentage of nanoparticles released in 24 hours was 10.35%, while the %EE values were 74.96 % and %DL 15.23 %. The IC50 value of zerumbone chitosan-folic acid nanoparticles was 14.270 µg/ml. Zerumbone chitosan-folic acid nanoparticles were successfully synthesized using the ionic gelation method, well characterized, and showed cytotoxicity toward MCF7 cells.