Multiphoton microscopy for the visualization of collagen fiber alignment and extracellular matrix remodeling in fibrous meningioma

Extracellular Matrix (ECM) play a key role in the growth and biomechanical properties of fibrous meningiomas. In particular, tumor cells' growth and invasive behavior may be impacted by the distribution and accumulation of collagen. In this study, we used Multiphoton Microscopy (MPM) for label-free imaging of fibrous meningiomas tissues. MPM based on two-photon fluorescence and second harmonic visualization of fibrous meningioma tumor cell morphological features and collagen fiber distribution around tumor cells. The results demonstrate that the MPM imaging technique enables the visualization of the intricate fibrous network structure formed by collagen surrounding tumor cells. This finding provides new insights into the role of collagen in fibrous meningiomas and further lays a scientific foundation for the development of personalized treatment strategies and novel diagnostic and therapeutic targets.