Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells
Understanding metabolic conditions related to glycolysis dependence is crucial for developing new treatments in cancer and regenerative medicine. This protocol details a method for using the live-cell metabolic analyzer (LiCellMo) to measure continuous changes in glucose consumption and lactate production in cultured human cells. LiCellMo provides real-time data on consecutive metabolic changes, improving measurements of these processes in various contexts, including in cancer and regenerative treatments.
Authors
- Koji Yoshimoto (ORCID: https://orcid.org/0000-0001-7912-2024)
- Takeshi Uchiumi (ORCID: https://orcid.org/0000-0002-3665-233X)
- Hiroki Kittaka
- Ko Igami
- Akio Tani (ORCID: https://orcid.org/0000-0001-7685-9063)
- Satoshi Fukumoto (ORCID: https://orcid.org/0000-0002-5025-6552)
- Mikako Yagi
- Kenji Miki (ORCID: https://orcid.org/0000-0001-7424-0021)
- Noboru Horiuchi
Institutions
- Kyushu University (JP)
- Ehime University (JP)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-01-10
- DOI
- https://doi.org/10.1016/j.xpro.2024.103518
- Citations
- 1
- Primary Topic
- Cancer, Hypoxia, and Metabolism
- Type
- article
- Field-Weighted Citation Impact
- 0.94
Funders
- Fukuoka Public Health Promotion Organization Cancer Research Fund
- Japan Society for the Promotion of Science London
- Stiftung für klinische Forschung zur Förderung der oralen Gesundheit
- Japan Society for the Promotion of Science