Prevention of Insulin Resistance by Herbal Compound from Fruit-Pulp of Eugenia jambolana in fructose fed rats

Aim: Present study was undertaken to investigate the preventive effect of active principle (FIIc) isolated from Eugenia jambolana on the development of insulin resistance induced by fructose in rats.Methodology: Crude aqueous extract of fruit-pulp of Eugenia jambolana was subjected to purification via Ion-exchange column chromatography that yielded FII which on further purification resulted FIIc.The purity of FIIc was tested by HPLC.FIIc was administered orally at a dose of 15 mg/kg b.wt.for 60 days to experimental rats.Body weight, blood glucose, serum triglyceride, total cholesterol, LDL-C, HDL-C, liver and muscle glycogen levels, TNF , serum insulin, insulin resistance and insulinogenic index were estimated at the interval of 30 days up to a period of 60 days.Results: Fructose feeding for 60 days resulted significant (p<0.001)increase in serum biochemical parameters and decreased liver and skeletal muscle glycogen levels in untreated control.Administration of FIIc showed significant (p<0.001)reduction in blood glucose when compared with untreated control.Body weight, lipid profile and liver and skeletal muscle glycogen levels were significantly (p<0.001)improved after treatment with FIIc.Levels of serum TNF and insulin were found to near normal following treatment with FIIc.Insulin resistance and insulinogenic index were significantly improved compared to untreated control.Conclusion: Our study showed that treatment with FIIc has a significant effect on fructose induced insulin resistance.