Reelin and S1P signaling contribute to horizontal-to-radial transitioning of new DGCs cooperatively

Adult neurogenesis is a process that brain generates new neuron and integrates them into the existing circuits. This process is regulated by many different factors. Reelin is one protein that plays an important role in adult neurogenesis. It is a secreted glycoprotein that can initiate the downstream signaling of the Reelin pathway, which promotes new neuron maturation and circuit integration. More recently, the S1P pathway has also been found to contribute to the maturation of new neurons. However, it is unknow if there is any correlation between these two pathways. To study their relationship, I will utilize shRNA knockdown techniques and fluorescent microscopy to assess the impact of the loss of their expression. The findings from this project may deepen our understanding of the mechanisms underlying adult neurogenesis.