Protocol to study neuronal membrane proteasome function in mouse peripheral sensory neurons
Neuronal membrane proteasomes (NMPs) are expressed on a subset of somatosensory dorsal root ganglion (DRG) neurons and influence mechanical and pain sensitivity. Here, we present a protocol for studying NMP function in mouse peripheral sensory neurons. We describe steps for procuring and culturing primary DRG neurons. We then detail biochemical and antibody feeding approaches to analyze NMP expression and localization. Finally, we include Ca<sup>2+</sup> imaging techniques for investigating NMP function in DRG neurons. For complete details on the use and execution of this protocol, please refer to Villalón Landeros et al.<sup>1</sup>.
Authors
- Eric Villalón (ORCID: https://orcid.org/0000-0003-2068-4094)
- Anna Marie Brennan (ORCID: https://orcid.org/0000-0003-3896-1985)
- Taylor R. Church (ORCID: https://orcid.org/0000-0001-6960-6236)
- Fulya Türker (ORCID: https://orcid.org/0000-0002-0441-090X)
- Emily R. Krueger
Institutions
- Johns Hopkins University (US)
- Bilkent University (TR)
- Loyola University Chicago (US)
- Johns Hopkins Medicine (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-01-10
- DOI
- https://doi.org/10.1016/j.xpro.2024.103552
- Primary Topic
- Neurobiology and Insect Physiology Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Loyola University Chicago
- National Institutes of Health
- Loyola University Chicago Stritch School of Medicine
- National Institute of Neurological Disorders and Stroke