Investigation of Matrix Effect in the Determination of Bisphenols in Aquatic Products by Liquid Chromatography-Mass Spectrometry

In this study, techniques such as liquid chromatography-ultraviolet photometric de- tector and evaporative light scattering detector were used to search for substances in the matrix that produced matrix effects when LC-MS was used to determine bisphenol compounds in aquatic products. This study provides new insights for researchers dealing with matrix effect problems. Bisphenols are introduced, with a focus on BPA and BPS. LC-MS is a commonly used method for the determination of bisphenols, but it is often affected by matrix effects, primarily due to phospholipids present in aquatic products. To confirm this, a chromatographic method was established to identify substances in the matrix that may co-elute with BPs. Phospholipids were confirmed as significant contrib- utors to matrix effects. Finally, commonly used methods to eliminate matrix effects are summarized