OPTIMIZATION OF NUTRIENT MEDIUM COMPOSITION FOR CLONAL MICROPROPAGATION OF FRAGARIA ANANASSA

The article presents the results of research on optimization of the composition of nutrient medium for effective replication of garden strawberry (Fragaria × ananassa) microplants. Garden strawberry is a highly demanded berry crop due to its high flavor and rich vitamin composition. Biological features of this crop create limiting factors for accelerated multiplication of high-yielding varieties and their introduction into production. The method of clonal micropropagation allows overcoming many problems of vegetatively propagated crops, including those related to stunting and disease incidence. In this study, the effect of agar-agar as a part of nutrient medium MS and dosage of BAP preparation on the dynamics of development of strawberry plants under in vitro conditions was investigated. The results of the study showed that for induction of strawberry (Fragaria × ananassa) morphogenesis under in vitro conditions, the use of liquid nutrient medium MS with the addition of BAP preparation at a dosage of 0.5 mg/L is the most effective, since the plant height growth, leaf and root formation are higher. Thus, using liquid nutrient medium with BAP preparation at a dosage of 0.5 mg/l, it is possible to obtain fully formed plants with well-developed root system and developed leaf apparatus in a short period of time. This is of great practical importance to accelerate the growth of strawberry starter material of valuable varieties, suitable for further propagation in production. Key words: strawberry, nutrient medium, development, in vitro, height, root formation.