Content of biologically active substances in aqueous extracts of bark-wood waste degradation products

The chemical composition of the destruction products of bark-wood waste (BWW) from the dump of JSC “Syktyvkar Timber Processing Company” was studied. The content of water-soluble fractions in samples from two wells ranged from 0.1 to 1.1% of the dry matter mass and depended on the depth of occurrence. These differences may be related to different time of occurrence, heterogeneity of waste, degree of decomposition and moisture content. Sequential treatment of the dry water-soluble residue with solvents of increasing polarity yielded hexane, ether and chloroform extracts. The content of dihydroquercetin (tR=28.6 min), vanillic acid (tR=11.6 min) and trans-p-coumaric acid (tR=17.7 min) were confirmed by TLC and HPLC methods. The above substances were previously detected in ethyl acetate and hexane extracts. Arabinose, mannose, glucose and galactose were detected when analyzing BWW samples for polysaccharide and rabinogalactan content in the hydrolysis products of water-soluble hemicellulose by HPLC. This allows us to characterize the isolated fraction as a mixture of water-soluble hemicelluloses – arabinogalactan and glucomannan. The same components – dihydroquercetin, vanillic and trans-p-coumaric acids – were found in all studied BWW samples regardless of the depth of occurrence and the use of water or organic solvents of different polarity for extraction. The detection of such strong antioxidants as dihydroquercetin, phenolic acids and arabinogalactan in BWW samples opens prospects for the development of substances based on them for medicine, animal husbandry and other sectors of national economy.