Engineered Focal Oncogene Amplifications Drive Transformation and Progression

Main Findings: Engineering oncogene-containing extrachromosomal DNA generates preclinical immunocompetent mouse models of cancer.Concept: Cre-mediated recombination of a region flanked by loxP sites of the same orientation enable circularization and subsequent amplification under selection.Impact: This study advances preclinical modeling of human cancer that will inform the understanding of focal amplification–driven tumorigenesis.